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padi2 polyclonal antibody  (Proteintech)


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    Structured Review

    Proteintech padi2 polyclonal antibody
    Padi2 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/padi2+polyclonal+antibody/PADI2+Antibody/pmc12966709-57-0-4
    Average 94 stars, based on 76 article reviews
    padi2 polyclonal antibody - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Peptidylarginine Deiminase Type 2 Predicts Tumor Progression and Poor Prognosis in Patients with Curatively Resected Biliary Tract Cancer
    Article Snippet: Immunohistochemical (IHC) staining was conducted using a Super SensitiveTM Polymer-HRP IHC detection system (Biogenex, San Ramon, CA, USA). .. The slides were incubated with PADI2 polyclonal antibody (12110-1-AP, Proteintech, Rosemont, IL, USA) at a dilution of 1:50 at 4 °C overnight, followed by a wash, and then incubated with the peroxidase-conjugated secondary antibody (Abcam, Cambridge, UK), showing cytoplasm and nucleus staining. ..

    Article Title: Peptidylarginine Deiminase Type 2 Predicts Tumor Progression and Poor Prognosis in Patients with Curatively Resected Biliary Tract Cancer.
    Article Snippet: Immunohistochemical (IHC) staining was conducted using a Super SensitiveTM Polymer-HRP IHC detection system (Biogenex, San Ramon, CA, USA). .. The slides were incubated with PADI2 polyclonal antibody (12110-1-AP, Proteintech, Rosemont, IL, USA) at a dilution of 1:50 at 4 °C overnight, followed by a wash, and then incubated with the peroxidase-conjugated secondary antibody (Abcam, Cambridge, UK), showing cytoplasm and nucleus staining. ..

    Article Title: Peptidylarginine Deiminase Type 2 Predicts Tumor Progression and Poor Prognosis in Patients with Curatively Resected Biliary Tract Cancer.
    Article Snippet: Immunohistochemical (IHC) staining was conducted using a Super SensitiveTM Polymer-HRP IHC detection system (Biogenex, San Ramon, CA, USA). .. The slides were incubated with PADI2 polyclonal antibody (12110-1-AP, Proteintech, Rosemont, IL, USA) at a dilution of 1:50 at 4 ◦C overnight, followed by a wash, and then incubated with the peroxidase-conjugated secondary antibody (Abcam, Cambridge, UK), sho ing cytoplasm and nucleus staining. ..

    Article Title: Macrophage extracellular traps as key mediators of scleral remodeling in myopia induced by hypoxia and activated platelets.
    Article Snippet: .. Samples were subjected to SDS-PAGE using 12.5%, 10%, or 7.5% gels (PG211, PG212, PG213, Epizyme Biotech, China) and transferred to polyvinylidene difluoride (PVDF) membranes (IPVH00010, Millipore, USA), which were subsequently blocked using Blocking Buffer (P0252, Beyotime, China) for 30 min at RT and incubated with primary antibodies: 1:1000 anti-citH3 antibody(ab219407, Abcam, UK), 1:1000 Collagen I/COL1A1 Rabbit pAb (A1352, Abclonal, China), 1:1000 Alpha smooth muscle actin Polyclonal antibody (14395-1-AP, Proteintech, China), 1:2000 HIF-1 alpha Polyclonal antibody (20960-1-AP, Proteintech, China), 1:5000 Anti-β-Tubulin(C66)mAb (M20005, Abmart, China), 1:1000 LC3B (D11) XP Rabbit mAb (3868, CST, USA), 1:5000 P62/SQSTM1 Polyclonal antibody (18420-1-AP, Proteintech, China), 1:1000 PADI4 Polyclonal antibody (17373-1-AP, Proteintech, China), 1:1000 PADI2 Polyclonal antibody (12110-1-AP, Proteintech, China), or 1:1000 β-Actin (13E5) Rabbit mAb (4970, CST, USA) at 4◦C overnight. .. The samples were then incubated for 1 h at RT with a 1:4000 dilution of Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H + L) (RGAR001, Proteintech, China) or Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H + L) (RGAM001, Proteintech, China).

    Staining:

    Article Title: Peptidylarginine Deiminase Type 2 Predicts Tumor Progression and Poor Prognosis in Patients with Curatively Resected Biliary Tract Cancer
    Article Snippet: Immunohistochemical (IHC) staining was conducted using a Super SensitiveTM Polymer-HRP IHC detection system (Biogenex, San Ramon, CA, USA). .. The slides were incubated with PADI2 polyclonal antibody (12110-1-AP, Proteintech, Rosemont, IL, USA) at a dilution of 1:50 at 4 °C overnight, followed by a wash, and then incubated with the peroxidase-conjugated secondary antibody (Abcam, Cambridge, UK), showing cytoplasm and nucleus staining. ..

    Article Title: Peptidylarginine Deiminase Type 2 Predicts Tumor Progression and Poor Prognosis in Patients with Curatively Resected Biliary Tract Cancer.
    Article Snippet: Immunohistochemical (IHC) staining was conducted using a Super SensitiveTM Polymer-HRP IHC detection system (Biogenex, San Ramon, CA, USA). .. The slides were incubated with PADI2 polyclonal antibody (12110-1-AP, Proteintech, Rosemont, IL, USA) at a dilution of 1:50 at 4 °C overnight, followed by a wash, and then incubated with the peroxidase-conjugated secondary antibody (Abcam, Cambridge, UK), showing cytoplasm and nucleus staining. ..

    Article Title: Peptidylarginine Deiminase Type 2 Predicts Tumor Progression and Poor Prognosis in Patients with Curatively Resected Biliary Tract Cancer.
    Article Snippet: Immunohistochemical (IHC) staining was conducted using a Super SensitiveTM Polymer-HRP IHC detection system (Biogenex, San Ramon, CA, USA). .. The slides were incubated with PADI2 polyclonal antibody (12110-1-AP, Proteintech, Rosemont, IL, USA) at a dilution of 1:50 at 4 ◦C overnight, followed by a wash, and then incubated with the peroxidase-conjugated secondary antibody (Abcam, Cambridge, UK), sho ing cytoplasm and nucleus staining. ..

    SDS Page:

    Article Title: Macrophage extracellular traps as key mediators of scleral remodeling in myopia induced by hypoxia and activated platelets.
    Article Snippet: .. Samples were subjected to SDS-PAGE using 12.5%, 10%, or 7.5% gels (PG211, PG212, PG213, Epizyme Biotech, China) and transferred to polyvinylidene difluoride (PVDF) membranes (IPVH00010, Millipore, USA), which were subsequently blocked using Blocking Buffer (P0252, Beyotime, China) for 30 min at RT and incubated with primary antibodies: 1:1000 anti-citH3 antibody(ab219407, Abcam, UK), 1:1000 Collagen I/COL1A1 Rabbit pAb (A1352, Abclonal, China), 1:1000 Alpha smooth muscle actin Polyclonal antibody (14395-1-AP, Proteintech, China), 1:2000 HIF-1 alpha Polyclonal antibody (20960-1-AP, Proteintech, China), 1:5000 Anti-β-Tubulin(C66)mAb (M20005, Abmart, China), 1:1000 LC3B (D11) XP Rabbit mAb (3868, CST, USA), 1:5000 P62/SQSTM1 Polyclonal antibody (18420-1-AP, Proteintech, China), 1:1000 PADI4 Polyclonal antibody (17373-1-AP, Proteintech, China), 1:1000 PADI2 Polyclonal antibody (12110-1-AP, Proteintech, China), or 1:1000 β-Actin (13E5) Rabbit mAb (4970, CST, USA) at 4◦C overnight. .. The samples were then incubated for 1 h at RT with a 1:4000 dilution of Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H + L) (RGAR001, Proteintech, China) or Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H + L) (RGAM001, Proteintech, China).

    Blocking Assay:

    Article Title: Macrophage extracellular traps as key mediators of scleral remodeling in myopia induced by hypoxia and activated platelets.
    Article Snippet: .. Samples were subjected to SDS-PAGE using 12.5%, 10%, or 7.5% gels (PG211, PG212, PG213, Epizyme Biotech, China) and transferred to polyvinylidene difluoride (PVDF) membranes (IPVH00010, Millipore, USA), which were subsequently blocked using Blocking Buffer (P0252, Beyotime, China) for 30 min at RT and incubated with primary antibodies: 1:1000 anti-citH3 antibody(ab219407, Abcam, UK), 1:1000 Collagen I/COL1A1 Rabbit pAb (A1352, Abclonal, China), 1:1000 Alpha smooth muscle actin Polyclonal antibody (14395-1-AP, Proteintech, China), 1:2000 HIF-1 alpha Polyclonal antibody (20960-1-AP, Proteintech, China), 1:5000 Anti-β-Tubulin(C66)mAb (M20005, Abmart, China), 1:1000 LC3B (D11) XP Rabbit mAb (3868, CST, USA), 1:5000 P62/SQSTM1 Polyclonal antibody (18420-1-AP, Proteintech, China), 1:1000 PADI4 Polyclonal antibody (17373-1-AP, Proteintech, China), 1:1000 PADI2 Polyclonal antibody (12110-1-AP, Proteintech, China), or 1:1000 β-Actin (13E5) Rabbit mAb (4970, CST, USA) at 4◦C overnight. .. The samples were then incubated for 1 h at RT with a 1:4000 dilution of Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H + L) (RGAR001, Proteintech, China) or Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H + L) (RGAM001, Proteintech, China).

    other:

    Article Title: Targeting peptidyl-arginine deiminase 4 suppresses SARS-CoV-2 replication and modulates the inflammatory response
    Article Snippet: PADI2 Polyclonal antibody , Proteintech , Cat# 12110-1-AP; RRID: AB_2159475.



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    Image Search Results


    Protein validation of key genes contributing to intestinal mucosal barrier recovery using the in-depth proteins

    Journal: iScience

    Article Title: Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients

    doi: 10.1016/j.isci.2023.106831

    Figure Lengend Snippet: Protein validation of key genes contributing to intestinal mucosal barrier recovery using the in-depth proteins

    Article Snippet: PADI2 antibody , Bioss , Cat# bs-11662R, RRID: AB_2934324.

    Techniques:

    Validation of identified potential biomarkers in clinical patients and correlation analysis on their expression levels and the degree of intestine functional recovery (A) mRNA expression levels of PADI2 , FAM162A , AQP8 and SULT1A1 at different timepoints of intestinal injury repair after surgery in patients. (B) Protein expression levels of PADI2, FAM162A, AQP8 and SULT1A1 at different timepoints of intestinal injury repair after surgery in patients. (C) AQP8 and SULT1A1 mRNA levels in patients with and without oral dietary intolerance 48 h after surgery and in patients with and without infectious complications 7 days after surgery. (D) Correlation analysis of the mRNA levels of AQP8 and SULT1A1 at 48 h after surgery and the time of passing flatus and stool. ∗p ≤0.05, ∗∗p ≤0.01, ∗∗∗p ≤0.001, ∗∗∗∗p ≤0.0001, one-way ANOVA with Tukey’s multiple comparisons test. Data are represented as mean ± standard deviation.

    Journal: iScience

    Article Title: Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients

    doi: 10.1016/j.isci.2023.106831

    Figure Lengend Snippet: Validation of identified potential biomarkers in clinical patients and correlation analysis on their expression levels and the degree of intestine functional recovery (A) mRNA expression levels of PADI2 , FAM162A , AQP8 and SULT1A1 at different timepoints of intestinal injury repair after surgery in patients. (B) Protein expression levels of PADI2, FAM162A, AQP8 and SULT1A1 at different timepoints of intestinal injury repair after surgery in patients. (C) AQP8 and SULT1A1 mRNA levels in patients with and without oral dietary intolerance 48 h after surgery and in patients with and without infectious complications 7 days after surgery. (D) Correlation analysis of the mRNA levels of AQP8 and SULT1A1 at 48 h after surgery and the time of passing flatus and stool. ∗p ≤0.05, ∗∗p ≤0.01, ∗∗∗p ≤0.001, ∗∗∗∗p ≤0.0001, one-way ANOVA with Tukey’s multiple comparisons test. Data are represented as mean ± standard deviation.

    Article Snippet: PADI2 antibody , Bioss , Cat# bs-11662R, RRID: AB_2934324.

    Techniques: Expressing, Functional Assay, Standard Deviation

    Journal: iScience

    Article Title: Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients

    doi: 10.1016/j.isci.2023.106831

    Figure Lengend Snippet:

    Article Snippet: PADI2 antibody , Bioss , Cat# bs-11662R, RRID: AB_2934324.

    Techniques: Expressing, Software

    Protein validation of key genes contributing to intestinal mucosal barrier recovery using the in-depth proteins

    Journal: iScience

    Article Title: Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients

    doi: 10.1016/j.isci.2023.106831

    Figure Lengend Snippet: Protein validation of key genes contributing to intestinal mucosal barrier recovery using the in-depth proteins

    Article Snippet: Primary antibodies used for western blotting were anti-PADI2 (Bioss, bs-11662R, 1:1000), anti-FAM162A (Abcam, ab122295, 1:1000), anti-AQP8 (Bioss, bs-6786R, 1:500), anti-SULT1A1 (Bioss, bs-6283R, 1:1000) and anti-GAPDH (Abmart, P30008 M, 1:4000).

    Techniques:

    Validation of identified potential biomarkers in clinical patients and correlation analysis on their expression levels and the degree of intestine functional recovery (A) mRNA expression levels of PADI2 , FAM162A , AQP8 and SULT1A1 at different timepoints of intestinal injury repair after surgery in patients. (B) Protein expression levels of PADI2, FAM162A, AQP8 and SULT1A1 at different timepoints of intestinal injury repair after surgery in patients. (C) AQP8 and SULT1A1 mRNA levels in patients with and without oral dietary intolerance 48 h after surgery and in patients with and without infectious complications 7 days after surgery. (D) Correlation analysis of the mRNA levels of AQP8 and SULT1A1 at 48 h after surgery and the time of passing flatus and stool. ∗p ≤0.05, ∗∗p ≤0.01, ∗∗∗p ≤0.001, ∗∗∗∗p ≤0.0001, one-way ANOVA with Tukey’s multiple comparisons test. Data are represented as mean ± standard deviation.

    Journal: iScience

    Article Title: Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients

    doi: 10.1016/j.isci.2023.106831

    Figure Lengend Snippet: Validation of identified potential biomarkers in clinical patients and correlation analysis on their expression levels and the degree of intestine functional recovery (A) mRNA expression levels of PADI2 , FAM162A , AQP8 and SULT1A1 at different timepoints of intestinal injury repair after surgery in patients. (B) Protein expression levels of PADI2, FAM162A, AQP8 and SULT1A1 at different timepoints of intestinal injury repair after surgery in patients. (C) AQP8 and SULT1A1 mRNA levels in patients with and without oral dietary intolerance 48 h after surgery and in patients with and without infectious complications 7 days after surgery. (D) Correlation analysis of the mRNA levels of AQP8 and SULT1A1 at 48 h after surgery and the time of passing flatus and stool. ∗p ≤0.05, ∗∗p ≤0.01, ∗∗∗p ≤0.001, ∗∗∗∗p ≤0.0001, one-way ANOVA with Tukey’s multiple comparisons test. Data are represented as mean ± standard deviation.

    Article Snippet: Primary antibodies used for western blotting were anti-PADI2 (Bioss, bs-11662R, 1:1000), anti-FAM162A (Abcam, ab122295, 1:1000), anti-AQP8 (Bioss, bs-6786R, 1:500), anti-SULT1A1 (Bioss, bs-6283R, 1:1000) and anti-GAPDH (Abmart, P30008 M, 1:4000).

    Techniques: Expressing, Functional Assay, Standard Deviation

    Journal: iScience

    Article Title: Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients

    doi: 10.1016/j.isci.2023.106831

    Figure Lengend Snippet:

    Article Snippet: Primary antibodies used for western blotting were anti-PADI2 (Bioss, bs-11662R, 1:1000), anti-FAM162A (Abcam, ab122295, 1:1000), anti-AQP8 (Bioss, bs-6786R, 1:500), anti-SULT1A1 (Bioss, bs-6283R, 1:1000) and anti-GAPDH (Abmart, P30008 M, 1:4000).

    Techniques: Expressing, Software

    Journal: iScience

    Article Title: Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients

    doi: 10.1016/j.isci.2023.106831

    Figure Lengend Snippet:

    Article Snippet: PADI2 antibody , Bioss , Cat# bs-11662R, RRID: AB_2934324.

    Techniques: Expressing, Software